The Technology Readiness Level (TRL) Assessment is a systematic method for evaluating the maturity and readiness of a technology or innovation, particularly in the context of early-stage startups. The importance of TRL Assessment lies in its ability to provide a structured framework for gauging the technological progress of a venture, aiding both entrepreneurs and investors in making informed decisions. The TRL Assessment aims to categorise a technology on a scale from TRL 1 (conceptualisation) to TRL 9 (full deployment), clearly indicating its development status and potential for commercialisation. This methodology is especially essential in early startup stages across various industries, notably in technology, biotech, and clean energy, where innovation’s viability and market acceptance are critical for success. By systematically assessing the technical risks and uncertainties associated with technology, TRL Assessment informs resource allocation, guides strategic planning, and influences investment decisions, ensuring that startups can effectively navigate the complexities of bringing cutting-edge innovations to market. Ultimately, TRL Assessment enhances communication between founders and investors, fostering a shared understanding of a startup’s technological readiness and its pathway towards commercialisation.